Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF-7 breast cancer cell line

cell line

MCF-7

cell type

Pleural effusion from an adenocarcinoma of the breast

treatment

Dexamethasone

concentration

100nM

time

30 minutes

replicate

replicate 1

antibody

GR E-20X sc-1003 Santa Cruz

growth proptocol

For maintenance cells were cultured in DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

48897477

Reads aligned (%)

96.4

Duplicates removed (%)

13.9

Number of peaks

13463 (qval < 1E-05)

hg38

Number of total reads

48897477

Reads aligned (%)

97.1

Duplicates removed (%)

12.7

Number of peaks

13553 (qval < 1E-05)