Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Others

Cell type

Tumor

NA

NA

Sample

source_name

Primary Patient Derived Tumor Model

tumor model

IN528

antibody

Rabbit anti-H3K4me1 Abcam #8895

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

H3K4me1ChIP was performed from 5x106 crosslinked p0-CSCs and DNA isolated as previously described (Rabbit anti-H3K4me1 Abcam #8895) (Corradin et al., 2014). Sequencing libraries were prepared as previously described (Corradin et al. 2014). ChIP-seq. ChIP-seq libraries were sequenced on the Illumina HiSeq 2500 platform at the Case Western Reserve University Genomics Core Facility.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

36605376

Reads aligned (%)

63.7

Duplicates removed (%)

47.3

Number of peaks

522 (qval < 1E-05)

hg19

Number of total reads

36605376

Reads aligned (%)

63.4

Duplicates removed (%)

47.6

Number of peaks

518 (qval < 1E-05)