Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Tfh

NA

NA

Sample

source_name

in vivo-generated T follicular helper (Tfh) cells from Bcl6-RFP reporter mice

strain

C57BL/6

chip antibody

IgG

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Bcl6-DNA complex was isolated by anti-Bcl6 antibody (SC858 (N-3), Santa Cruz) Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

36564979

Reads aligned (%)

86.1

Duplicates removed (%)

45.7

Number of peaks

62161 (qval < 1E-05)

mm9

Number of total reads

36564979

Reads aligned (%)

85.5

Duplicates removed (%)

45.5

Number of peaks

62141 (qval < 1E-05)