Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CUTLL1
NA
NA

Attributes by original data submitter

Sample

source_name
CUTLL1, ICN1, H3K27ac ChIP
cell line
CUTLL1
cell type
T-cell lymphoma cell line
notch
wild-type
chip antibody
H3K27ac

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with antibody. Libraries were prepared according to New England BioLabs instructions accompanying the DNA Sample Prep Master Mix Set 1 (NEB E6040S).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
49815836
Reads aligned (%)
89.1
Duplicates removed (%)
20.2
Number of peaks
37986 (qval < 1E-05)

hg19

Number of total reads
49815836
Reads aligned (%)
88.8
Duplicates removed (%)
20.4
Number of peaks
38040 (qval < 1E-05)

Base call quality data from DBCLS SRA