Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MDA-MB-231

genotype/variation

Ty1-TA1del expressed

passages

3-7

antibody

anti-H3K4me1 (ab8895)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were prepared according to Illumina or BIOO SCIENTIFIC instruction. ATAC-seq libraries were prepared by following the original protocol (Buenrostro et al., 2013) with several modifications (as described above)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

43858667

Reads aligned (%)

95.6

Duplicates removed (%)

2.8

Number of peaks

41512 (qval < 1E-05)

hg19

Number of total reads

43858667

Reads aligned (%)

95.2

Duplicates removed (%)

2.9

Number of peaks

41187 (qval < 1E-05)