Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA3

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231
genotype/variation
GATA3 expressed
passages
3-7
antibody
GATA3 serum (Adomas et al, 2014)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to Illumina or BIOO SCIENTIFIC instruction. ATAC-seq libraries were prepared by following the original protocol (Buenrostro et al., 2013) with several modifications (as described above)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
20933891
Reads aligned (%)
166.5
Duplicates removed (%)
14.6
Number of peaks
28016 (qval < 1E-05)

hg38

Number of total reads
20933891
Reads aligned (%)
168.2
Duplicates removed (%)
14.3
Number of peaks
28263 (qval < 1E-05)

Base call quality data from DBCLS SRA