GSM1855848: (+) MET Input DNA rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116
cell type
human colon cancer
chip antibody
None
culture media
(+) Methionine
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells grown on 15cm plates were crosslinked with 1% formaldyhyde in culture media. Cross-linked chromatin was collected and flash frozen before sonication with the Covaris M2. Lysates were clarified and protein-DNA complexes were immunoprecipitated with H3K4me3 antibody. Libraries were prepared according to standard Illumina protocols