GSM1853824: PRDM9C-G278A H3K4me3 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293
cell description
embryonic kidney
prdm9 allele
PRDM9C-G278A
ChIP
H3K4me3
antibody manufacturer
Millipore/EMD
antibody catalog number
07-473
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated from cells using hypotonic lysis. Chromatin was fragmented by Mnase digestion. After high speed spin soluble chromatin was removed and used for ChIP Kapa Hyper Prep Kits (Kapa Biosystems)