Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Follicular B cells
strain
C57BL/6JNimr
tissue
Spleen
age
7-9 weeks
Sex
female
immunization
None
flow strategy
B220+AA4.1-Cd23+IgMintermediate
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Female mouse C57BL/6 B cell populations were sorted on BD FACSAriaII, BD Influx or Beckman Coulter MoFlo XDP cell sorters; collected cells were centrifuged and cross-linked with 1% formaldehyde and sonicated to shear the chromatin to ~500bp fragments; DNA was isolated with antibodies and purified by phenol-chloroform extraction Libraries were prepared with Illumina's DNA Sample prep kit according to the manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
30713029
Reads aligned (%)
98.8
Duplicates removed (%)
13.9
Number of peaks
457 (qval < 1E-05)

mm9

Number of total reads
30713029
Reads aligned (%)
98.6
Duplicates removed (%)
13.9
Number of peaks
470 (qval < 1E-05)

Base call quality data from DBCLS SRA