Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM3B

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Human colorectal cancer HCT116 cells
cell line
HCT116
cell type
Human colorectal cancer
chip antibody
KDM3B (Millipore, 09-816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Ovation Ultralow DR Multiplex System )

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
24291816
Reads aligned (%)
72.6
Duplicates removed (%)
17.6
Number of peaks
1605 (qval < 1E-05)

hg38

Number of total reads
24291816
Reads aligned (%)
74.0
Duplicates removed (%)
16.4
Number of peaks
1633 (qval < 1E-05)

Base call quality data from DBCLS SRA