Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUND

Cell type

Cell type Class
Epidermis
Cell type
Melanoma
MeSH Description
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)

Attributes by original data submitter

Sample

source_name
metastasis of cutaneous melanoma
cell type
patient derived xenograft cells
passage
2
melanoma mutation
NRASQ61K
infected shrna
shLuc
chip antibody
KMT2D (Sigma, HPA035977)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
20908138
Reads aligned (%)
70.6
Duplicates removed (%)
22.0
Number of peaks
6800 (qval < 1E-05)

hg38

Number of total reads
20908138
Reads aligned (%)
71.2
Duplicates removed (%)
21.6
Number of peaks
6769 (qval < 1E-05)

Base call quality data from DBCLS SRA