Antigen

Antigen Class

Histone

Antigen

H3K9me2

Cell type

Cell type Class

Blood

Cell type

Granulocytes

MeSH Description

Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.

Sample

source_name

Granulocyte from blood

sample id

Granulocyte 3

gender

female

chip antibody

H3K9me2 (ab1220 Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

cells were fixed with 1% formaldehyde for 10 min. After fixation, nuclei were isolated, the nuclear DNA was digested by micrococcal nuclease to 150-200 bp DNA fragments, the DNA fragments with attached proteins were resuspended in SDS-containing lysis buffer, and immunoprecipitated by antibodies against various histone modifications. For Illumina Hi-Seq 2500 sequencing we used New England BioLabs NEBNext ChIP-Seq library preparation reagent set for Illumina and and constructed library as described in the E6240 instruction manual. For SOLiD sequencing we used Applied Biosystems SOLiD 4 System and constructed library as described in the SOLiD 4 System Library Preparation Guide.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31125259

Reads aligned (%)

100.0

Duplicates removed (%)

11.7

Number of peaks

53424 (qval < 1E-05)

hg19

Number of total reads

31125259

Reads aligned (%)

100.0

Duplicates removed (%)

11.9

Number of peaks

53636 (qval < 1E-05)