Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
Preimplantation embryo
NA
NA

Attributes by original data submitter

Sample

source_name
Preimplantation embryo
strain
C57BL/6N x PWK
developmental stage
Preimplantation embryo
chip antibody
custom-made H3K4Me3 antibody (Abmart)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
57493490
Reads aligned (%)
64.0
Duplicates removed (%)
34.5
Number of peaks
4279 (qval < 1E-05)

mm9

Number of total reads
57493490
Reads aligned (%)
63.8
Duplicates removed (%)
34.5
Number of peaks
4233 (qval < 1E-05)

Base call quality data from DBCLS SRA