Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4;11_SGCwashout_ChIP
tissue
peripheral blood
cell line
MV4;11
cell type
Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line
treatment
5uM SGC0946 for 8 hrs and washout
chip antibody
anti-RNA Pol II (CTD4H8, Millipore, lot 2491218)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
19839354
Reads aligned (%)
97.2
Duplicates removed (%)
56.2
Number of peaks
5046 (qval < 1E-05)

hg19

Number of total reads
19839354
Reads aligned (%)
96.6
Duplicates removed (%)
57.0
Number of peaks
5064 (qval < 1E-05)

Base call quality data from DBCLS SRA