GSM1845169: SGCwashout Pol2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
MV4;11_SGCwashout_ChIP
tissue
peripheral blood
cell line
MV4;11
cell type
Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line
treatment
5uM SGC0946 for 8 hrs and washout
chip antibody
anti-RNA Pol II (CTD4H8, Millipore, lot 2491218)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)