Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4;11_SGCwashout_ChIP
tissue
peripheral blood
cell line
MV4;11
cell type
Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line
treatment
5uM SGC0946 for 8 hrs and washout
chip antibody
anti-BRD4 (E2A7X, cell signalling, lot 1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
19577183
Reads aligned (%)
88.6
Duplicates removed (%)
9.0
Number of peaks
11365 (qval < 1E-05)

hg38

Number of total reads
19577183
Reads aligned (%)
90.9
Duplicates removed (%)
7.4
Number of peaks
11436 (qval < 1E-05)

Base call quality data from DBCLS SRA