Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K79me2

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4;11_IBET+SGC_ChIP
tissue source
peripheral blood
cell line
MV4;11
cell type
Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line
treatment
500nM I-BET151 and 5uM SGC0946
chip antibody
anti-H3K79me2 (ab3594,abcam, lot GR201271-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
15976980
Reads aligned (%)
99.5
Duplicates removed (%)
3.2
Number of peaks
7013 (qval < 1E-05)

hg19

Number of total reads
15976980
Reads aligned (%)
99.3
Duplicates removed (%)
3.5
Number of peaks
7065 (qval < 1E-05)

Base call quality data from DBCLS SRA