Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

MV-4-11

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MV4;11_SGC_ChIP

tissue source

peripheral blood

cell line

MV4;11

cell type

Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line

treatment

5uM SGC0946

chip antibody

anti-RNA Pol II (CTD4H8, Millipore, lot 2491218)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

22960543

Reads aligned (%)

95.7

Duplicates removed (%)

19.2

Number of peaks

11634 (qval < 1E-05)

hg19

Number of total reads

22960543

Reads aligned (%)

95.1

Duplicates removed (%)

19.8

Number of peaks

11599 (qval < 1E-05)