Antigen

Antigen Class

Histone

Antigen

H3K79me2

Cell type

Cell type Class

Blood

Cell type

MV-4-11

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MV4;11_SGC_ChIP

tissue source

peripheral blood

cell line

MV4;11

cell type

Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line

treatment

5uM SGC0946

chip antibody

anti-H3K79me2 (ab3594,abcam, lot GR201271-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

15811856

Reads aligned (%)

99.6

Duplicates removed (%)

2.9

Number of peaks

6460 (qval < 1E-05)

hg19

Number of total reads

15811856

Reads aligned (%)

99.4

Duplicates removed (%)

3.1

Number of peaks

6493 (qval < 1E-05)