Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4;11_DMSO_ChIP
tissue source
peripheral blood
cell line
MV4;11
cell type
Mixed lineage leukemia (MLL) fusion protein (FP) leukemia cell line
treatment
0.1% DMSO
chip antibody
anti-H3K27ac (ab4729, abcam, lot GR85490-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
18670758
Reads aligned (%)
99.6
Duplicates removed (%)
9.3
Number of peaks
44548 (qval < 1E-05)

hg19

Number of total reads
18670758
Reads aligned (%)
99.3
Duplicates removed (%)
9.5
Number of peaks
44379 (qval < 1E-05)

Base call quality data from DBCLS SRA