Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
adult ovary
developmental stage
4-6 days old adult females
tissue
ovary
germline knockdown and other transgenes
aubsh1 attp40 ago3 sh attP2
chip or ip antibody
rabbit anti-rbp3 (custom made)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed according to Lee et al, Nat Protoc, 2006 ChIP-seq libraries were generated using the NEBNext™ DNA Sample Prep Reagent Set 1 for Illumina (NEB) and the Kapa HiFi Library Amp Real-Time Kit (Peqlab) for amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
24240947
Reads aligned (%)
92.2
Duplicates removed (%)
60.6
Number of peaks
5496 (qval < 1E-05)

dm3

Number of total reads
24240947
Reads aligned (%)
92.2
Duplicates removed (%)
59.4
Number of peaks
5762 (qval < 1E-05)

Base call quality data from DBCLS SRA