Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
adult ovary
developmental stage
4-6 days old adult females
tissue
ovary
germline knockdown and other transgenes
white sh attP2 (control)
chip or ip antibody
rabbit anti-rbp3 (custom made)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed according to Lee et al, Nat Protoc, 2006 ChIP-seq libraries were generated using the NEBNext™ DNA Sample Prep Reagent Set 1 for Illumina (NEB) and the Kapa HiFi Library Amp Real-Time Kit (Peqlab) for amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
12445702
Reads aligned (%)
91.0
Duplicates removed (%)
56.9
Number of peaks
5645 (qval < 1E-05)

dm3

Number of total reads
12445702
Reads aligned (%)
91.1
Duplicates removed (%)
56.0
Number of peaks
5785 (qval < 1E-05)

Base call quality data from DBCLS SRA