Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Adult

Cell type

Ovary

NA

NA

Sample

source_name

adult ovary

developmental stage

4-6 days old adult females

tissue

ovary

germline knockdown and other transgenes

white sh attP2 (control)

chip or ip antibody

rabbit anti-rbp3 (custom made)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to Lee et al, Nat Protoc, 2006 ChIP-seq libraries were generated using the NEBNext™ DNA Sample Prep Reagent Set 1 for Illumina (NEB) and the Kapa HiFi Library Amp Real-Time Kit (Peqlab) for amplification.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

11854057

Reads aligned (%)

90.0

Duplicates removed (%)

72.3

Number of peaks

4595 (qval < 1E-05)

dm3

Number of total reads

11854057

Reads aligned (%)

90.0

Duplicates removed (%)

71.3

Number of peaks

4737 (qval < 1E-05)