Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

D15-2864

cell line

Kasumi-1

time point

72 hours

chip antibody

H3K4me2

chip antibody vendor

Millipore

chip antibody vendor id

07-030

agent

DMSO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Treated cells in culture were cross-linked with 1% formaldehyde for 10 minutes and then queched with glycine. Washed cells were lysed and sonicated, followed by antibody immunoprecipitation. DNA libraries for ChIP-seq were prepared using Ovation Ultralow DR Multiplex System kits (0330-32, NuGEN) followed by Illumina sequencing at the MIT BioMicro Center.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

71800862

Reads aligned (%)

96.2

Duplicates removed (%)

6.3

Number of peaks

81900 (qval < 1E-05)

hg19

Number of total reads

71800862

Reads aligned (%)

95.6

Duplicates removed (%)

7.2

Number of peaks

82763 (qval < 1E-05)