Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
Kasumi-1

Cell type information


Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by Original Data Submitter


source_name
D15-2864
cell line
Kasumi-1
time point
72 hours
chip antibody
H3K4me2
chip antibody vendor
Millipore
chip antibody vendor id
07-030
agent
DMSO

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Treated cells in culture were cross-linked with 1% formaldehyde for 10 minutes and then queched with glycine. Washed cells were lysed and sonicated, followed by antibody immunoprecipitation. DNA libraries for ChIP-seq were prepared using Ovation Ultralow DR Multiplex System kits (0330-32, NuGEN) followed by Illumina sequencing at the MIT BioMicro Center.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
71800862
Reads aligned (%)
95.6
Duplicates removed (%)
7.2
Number of peaks
82763 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA