Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM1A

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
D15-2863
cell line
Kasumi-1
time point
72 hours
chip antibody
KDM1A
chip antibody vendor
Bethyl
chip antibody vendor id
A300-215A
agent
GSK690

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Treated cells in culture were cross-linked with 1% formaldehyde for 10 minutes and then queched with glycine. Washed cells were lysed and sonicated, followed by antibody immunoprecipitation. DNA libraries for ChIP-seq were prepared using Ovation Ultralow DR Multiplex System kits (0330-32, NuGEN) followed by Illumina sequencing at the MIT BioMicro Center.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
73874677
Reads aligned (%)
65.8
Duplicates removed (%)
32.2
Number of peaks
8757 (qval < 1E-05)

hg38

Number of total reads
73874677
Reads aligned (%)
67.7
Duplicates removed (%)
30.9
Number of peaks
8606 (qval < 1E-05)

Base call quality data from DBCLS SRA