Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM1A

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
D15-2862
cell line
Kasumi-1
time point
72 hours
chip antibody
KDM1A
chip antibody vendor
Bethyl
chip antibody vendor id
A300-215A
agent
RN-1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Treated cells in culture were cross-linked with 1% formaldehyde for 10 minutes and then queched with glycine. Washed cells were lysed and sonicated, followed by antibody immunoprecipitation. DNA libraries for ChIP-seq were prepared using Ovation Ultralow DR Multiplex System kits (0330-32, NuGEN) followed by Illumina sequencing at the MIT BioMicro Center.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
61754011
Reads aligned (%)
72.5
Duplicates removed (%)
25.4
Number of peaks
10393 (qval < 1E-05)

hg38

Number of total reads
61754011
Reads aligned (%)
74.4
Duplicates removed (%)
24.1
Number of peaks
10498 (qval < 1E-05)

Base call quality data from DBCLS SRA