Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Breast
Cell type
SUM 1315M02
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
SUM1315
cell line
SUM1315
chip antibody
BRD4 (Bethyl, catalog# A301-985A, lot# 4)
drug treatment
DMSO
duration
12 hour

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
58878104
Reads aligned (%)
45.0
Duplicates removed (%)
53.4
Number of peaks
9108 (qval < 1E-05)

hg38

Number of total reads
58878104
Reads aligned (%)
48.9
Duplicates removed (%)
50.0
Number of peaks
7021 (qval < 1E-05)

Base call quality data from DBCLS SRA