GSM1835984: ChIP-seq HCT116 Homozygous H3K27Ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
ChIP-seq_HCT116_Homozygous_H3K27Ac
cell line
HCT116
cell type
colorectal carcinoma derived cell line
genotype/variation
Homozygous ARID1A-KO
chip antibody
H3K27ac (Cell Signaling Technology, D5E4, 8173)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Cat.# IP-202-1012)