Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Kidney
Cell type
G-401
Primary Tissue
Kidney
Tissue Diagnosis
Wilms' tumor

Attributes by original data submitter

Sample

source_name
ChIP-seq_G401_Dox
cell line
G401
cell type
kidney rhabdoid tumor derived cell line
treatment/condition
Doxycycline induced SMARCB1 reexpresion
chip antibody
SMARCA4/BRG1 (Abcam, EPNCIR111A, ab110641)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Cat.# IP-202-1012)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
26069071
Reads aligned (%)
30.3
Duplicates removed (%)
2.8
Number of peaks
335 (qval < 1E-05)

hg38

Number of total reads
26069071
Reads aligned (%)
30.7
Duplicates removed (%)
2.1
Number of peaks
278 (qval < 1E-05)

Base call quality data from DBCLS SRA