Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Leukemic blast cell

NA

NA

Sample

source_name

Cultured Leukemic Blasts

chip antibody

H3K27ac (ActiveMotif, ab4729)

tissue

Cultured Leukemic Blasts

treatment compound

GSI BMS-906024

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin from formaldehyde-fixed cells (5 × 106 cells per histone mark) was fragmented to a size range of 200–700 bases with a Branson 250 Sonifier. Solubilized chromatin was immunoprecipitated with an antibody against H3K27ac (2.5 μl; ActiveMotif, ab4729), and enrichment for H3K27ac was confirmed by dot blot as described (Ernst et al. 2011). Antibody-chromatin complexes were pulled down with protein G magnetic beads (Dynabead, 10003D), washed and then eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was treated with RNase and purified with Agencourt AMPure XP (Beckman Coulter A63880). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

55169844

Reads aligned (%)

97.2

Duplicates removed (%)

83.9

Number of peaks

10792 (qval < 1E-05)

hg19

Number of total reads

55169844

Reads aligned (%)

96.6

Duplicates removed (%)

84.7

Number of peaks

10704 (qval < 1E-05)