Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Adult
Cell type
Ovary

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
adult fly ovaries
age
3-5 days old
chip antibody
none
tissue
adult fly ovaries

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody against H3K9me3 (ab8898). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, Taq DNA polymerase and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Taq DNA polymerase and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers for 15-18 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Platform Information


instrument_model
Illumina MiSeq

External Database Query

Logs in read processing pipeline


Number of total reads
4006265
Reads aligned (%)
93.5
Duplicates removed (%)
15.9
Number of peaks
96 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA