Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
prostate tumor
cell line
MCF7
transgene
HOTAIR
treatment
E2
antibody
ER

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiScanSQ

hg38

Number of total reads
12432968
Reads aligned (%)
94.1
Duplicates removed (%)
10.7
Number of peaks
12733 (qval < 1E-05)

hg19

Number of total reads
12432968
Reads aligned (%)
93.6
Duplicates removed (%)
11.3
Number of peaks
12703 (qval < 1E-05)

Base call quality data from DBCLS SRA