Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

prostate tumor

cell line

MCF7

transgene

GFP

treatment

E2

antibody

ER

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiScanSQ

hg38

Number of total reads

13010384

Reads aligned (%)

96.3

Duplicates removed (%)

11.6

Number of peaks

10286 (qval < 1E-05)

hg19

Number of total reads

13010384

Reads aligned (%)

95.8

Duplicates removed (%)

12.3

Number of peaks

10277 (qval < 1E-05)