Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Adult

Cell type

Whole body

NA

NA

Sample

source_name

Adult male fly

tissue

Whole Body

Sex

male

developmental stage

adult

genetic background

atf3 mutant

transfection

Atf3::GFP fusion protein

chip antibody

GFP-Trap_A (gta-20, Chromotek, Germany)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Atf3-DNA complexes were isolated with agarose beads coupled to anti-GFP antibody Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

27426213

Reads aligned (%)

96.2

Duplicates removed (%)

12.2

Number of peaks

1916 (qval < 1E-05)

dm3

Number of total reads

27426213

Reads aligned (%)

96.8

Duplicates removed (%)

10.2

Number of peaks

0 (qval < 1E-05)