Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Adult
Cell type
Whole body
NA
NA

Attributes by original data submitter

Sample

source_name
Adult male fly
tissue
Whole Body
Sex
male
developmental stage
adult
genetic background
atf3 mutant
transfection
--
chip antibody
GFP-Trap_A (gta-20, Chromotek, Germany)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Atf3-DNA complexes were isolated with agarose beads coupled to anti-GFP antibody Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
27945773
Reads aligned (%)
95.9
Duplicates removed (%)
13.6
Number of peaks
2057 (qval < 1E-05)

dm3

Number of total reads
27945773
Reads aligned (%)
96.7
Duplicates removed (%)
11.7
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA