NSD3 (polyclonal antibody made in house raised against the peptide: PTDYYHSEIPNTRPHEC)
cell type
Acute myeloid leukemia
genotype
MLL-AF9/NrasG12D
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with appopriate sizes were prepared for sequencing. ChIP-Seq libraries were constructed with TruSeq ChIP Sample Prep Kit (Illumina, San Diego, CA) according to the manufacturer’s instructions.The quantity and quality of all libraries were determined by Bioanalyzer with high-sensitivity chips.