Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Gonad
Cell type
OVCAR-3
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Ovarian cancer cell line OVCAR3
cell line
Ovarian cancer cell line OVCAR3
chip antibody
GRHL2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. a ChIP-seq library was constructed using NEBNext® ChIP-seq sample preparation kit (E6200, New England Biolabs) according to the manufacturer’s protocol. Size-selection was performed by gel extraction after agarose gel electrophoresis or by magnetic separation using AMPure XP beads. Multiplexing sample preparation oligonucleotide kit (#PE-400-1001, Illumina) was used for index labelling. Sample quality was analyzed using an Agilent 2100 Bioanalyzer prior to sequencing.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
16902736
Reads aligned (%)
80.4
Duplicates removed (%)
6.3
Number of peaks
3823 (qval < 1E-05)

hg38

Number of total reads
16902736
Reads aligned (%)
81.2
Duplicates removed (%)
5.9
Number of peaks
3705 (qval < 1E-05)

Base call quality data from DBCLS SRA