GSM1825676: OVCAR3-GRHL2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GRHL2
Cell type
Cell type Class
Gonad
Cell type
OVCAR-3
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Ovarian cancer cell line OVCAR3
cell line
Ovarian cancer cell line OVCAR3
chip antibody
GRHL2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. a ChIP-seq library was constructed using NEBNext® ChIP-seq sample preparation kit (E6200, New England Biolabs) according to the manufacturer’s protocol. Size-selection was performed by gel extraction after agarose gel electrophoresis or by magnetic separation using AMPure XP beads. Multiplexing sample preparation oligonucleotide kit (#PE-400-1001, Illumina) was used for index labelling. Sample quality was analyzed using an Agilent 2100 Bioanalyzer prior to sequencing.