Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gfi1

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Gfi1-KO CD4 Thn cell
strain background
C57BL/6
genotype/variation
Gfi1-dficient
tissue
spleen
cell type
Naive CD4 T (CD44lowCD62LhighCD25negative) cells
chip antibody
Gfi
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and transcription factor-DNA complexes were isolated with antibody. Libraries were prepared according to NEB's instructions accompanying the NEBNext ChIP-Seq Library prep regent (E6200)

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
26747144
Reads aligned (%)
52.8
Duplicates removed (%)
37.4
Number of peaks
980 (qval < 1E-05)

mm9

Number of total reads
26747144
Reads aligned (%)
52.7
Duplicates removed (%)
37.6
Number of peaks
1051 (qval < 1E-05)

Base call quality data from DBCLS SRA