Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Others
Cell type
TC-797
NA
NA

Attributes by original data submitter

Sample

source_name
797
cell type
NMC
cell line
797_B4N_C
pulldown with biotap_tag
C- BioTAP-BRD4-NUT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1.5 × 109 cells grown as monolayer cultures in 150mm dishes (100 plates total) were used for BioTAP-XL purification. Tetracycline was added (1 μg/mL) to 60-70 % confluent culture to induce transcription of the N- or C-BioTAP tagged BRD4-NUT cDNA clones for 16h. The main steps of BioTAP-XL procedure: harvesting cells, formaldehyde cross-linking, chromatin preparation, affinity purification, input and IP DNA recovery, and ChIP-seq library preparation, were performed as described (Alekseyenko et al. 2014a; Alekseyenko et al. 2015) ChIP-seq library preparation, were performed as described (Alekseyenko et al. 2014a; Alekseyenko et al. 2015)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
5886506
Reads aligned (%)
99.4
Duplicates removed (%)
0.5
Number of peaks
250 (qval < 1E-05)

hg19

Number of total reads
5886506
Reads aligned (%)
99.1
Duplicates removed (%)
0.5
Number of peaks
257 (qval < 1E-05)

Base call quality data from DBCLS SRA