GSM1820915: Input 797 B4N N; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Others
Cell type
TC-797
NA
NA
Attributes by original data submitter
Sample
source_name
797
cell type
NMC
cell line
797_B4N_N
pulldown with biotap_tag
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
1.5 × 109 cells grown as monolayer cultures in 150mm dishes (100 plates total) were used for BioTAP-XL purification. Tetracycline was added (1 μg/mL) to 60-70 % confluent culture to induce transcription of the N- or C-BioTAP tagged BRD4-NUT cDNA clones for 16h. The main steps of BioTAP-XL procedure: harvesting cells, formaldehyde cross-linking, chromatin preparation, affinity purification, input and IP DNA recovery, and ChIP-seq library preparation, were performed as described (Alekseyenko et al. 2014a; Alekseyenko et al. 2015) ChIP-seq library preparation, were performed as described (Alekseyenko et al. 2014a; Alekseyenko et al. 2015)