Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Digestive tract
Cell type
Intestinal stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Control mouse intestinal stem cells
strain
B6; 129 mixed background
cell type
Adult intestinal stem cell; proximal 2/3 of intestine
genotype
Cdx2+/+; Lgr5-EGFP-IRES-CREERT2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell lysates were sonicated and incubated with 3 ug CDX2 antibody overnight (Bethyl Labs; A300-691A) for immunoprecipitation. The ThruPLEX-FD Prep Kit (Rubicon Genomics) was used to prepare libraries, and 50 bp single-end reads were sequenced on an Illumina Hi-Seq 2000 instrument.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
149257007
Reads aligned (%)
98.4
Duplicates removed (%)
57.2
Number of peaks
989 (qval < 1E-05)

mm9

Number of total reads
149257007
Reads aligned (%)
98.2
Duplicates removed (%)
57.2
Number of peaks
1039 (qval < 1E-05)

Base call quality data from DBCLS SRA