GSM1817657: ChIP-Re ChIP K562 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Leukemia Chronic Myelogenous
Attributes by original data submitter
Human chronic myelogenous leukemia; lymphoblastoid cells.
First, IP with custom mouse monoclonal BORIS Ab, next IP with custom mouse monoclonal CTCF
Sequenced DNA Library
After overnight incubation with DiaMag magnetic beads (Diagenode, Inc.) and CTCF or BORIS monoclonal or polyclonal antibodies (characterized and described by PMID:15731119; PMID: 21659515; PMID:20231363), precipitated chromatin was then washed, decrosslinked, and digested with proteinase K. The resulting DNA was purified using phenol/chloroform/isoamyl alcohol. DNA concentration was assessed with Quant‐it PicoGreen dsDNA kit (Invitrogen) and 5–10 ng was used to generate sequencing libraries. TruSeq ChIP Sample Preparation Kit (Illumina, Inc., USA). RNA-seq libraries were prepared using either Ion Total RNA-seq v2 kit or TruSeq Stranded RNA LT Kit (Illumina).