Antigen

Antigen Class

Histone

Antigen

H3K9ac

Cell type

Cell type Class

Lung

Cell type

Carcinoma, Lewis Lung

MeSH Description

A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.

Sample

source_name

drug sensitive Lewis lung carcinoma isograft

cell type

LLC-NR

phenotype

drug sensitive

tissue

lung

chip antibody

H3K9ac (Abcam, #ab10812)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Tissue homogenate were harvested for extraction of nuclei and digested with micrococcal nuclease. Then histone modification antibodies (i.e. H3K4me3,H3K27me3,H3K36me3,H3K9ac) were used to immunoprecipitate the chromatin. Libraries were prepared for sequencing using standard Illumina HiSeq protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

58276318

Reads aligned (%)

97.4

Duplicates removed (%)

14.2

Number of peaks

476 (qval < 1E-05)

mm9

Number of total reads

58276318

Reads aligned (%)

97.3

Duplicates removed (%)

14.2

Number of peaks

496 (qval < 1E-05)