Antigen

Antigen Class

TFs and others

Antigen

NR2F1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived neural crests

NA

NA

Sample

source_name

Cranial neural crest cells

individual

1

cell type

Cranial neural crest cells (CNCCs)

cnccs derived from

H9 (ESC)

chip antibody

anti-NR2F1 (Perseus Proteomics, catalog# PP-H8132-00, lot# A-2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPs were performed using approximately 0.5-1 x 10^7 CNCCs fixed with 1% formaldehyde for 10 min at room temp. RNA was extracted using Trizol. Sequencing libraries were prepared starting from ~30ng of ChIP DNA or oligo-dT purified mRNA using the NEBNext Multiplex Oligos for Illumina kit (Cat# E7335S).

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

32715879

Reads aligned (%)

98.5

Duplicates removed (%)

22.7

Number of peaks

74106 (qval < 1E-05)

hg19

Number of total reads

32715879

Reads aligned (%)

98.3

Duplicates removed (%)

23.2

Number of peaks

73935 (qval < 1E-05)