Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Irf8

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
GC B cells
cell type
GC B cells
strain
C57BL/6
culture time
in vivo fresh cell
chip antibody
anti-IRF8 (Santa Cruz, C-19)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody Libraries were prepared for sequencing usingTruSeq DNA LT Sample Prep Kit with standard protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
56753118
Reads aligned (%)
82.9
Duplicates removed (%)
79.1
Number of peaks
2285 (qval < 1E-05)

mm9

Number of total reads
56753118
Reads aligned (%)
82.7
Duplicates removed (%)
79.2
Number of peaks
2231 (qval < 1E-05)

Base call quality data from DBCLS SRA