Antigen

Antigen Class

TFs and others

Antigen

Irf8

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

LPS actived B cells

cell type

LPS actived B cells

strain

C57BL/6

culture time

48 hours

chip antibody

anti-IRF8 (Santa Cruz, C-19)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody Libraries were prepared for sequencing usingTruSeq DNA LT Sample Prep Kit with standard protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

14974564

Reads aligned (%)

95.3

Duplicates removed (%)

72.3

Number of peaks

313 (qval < 1E-05)

mm9

Number of total reads

14974564

Reads aligned (%)

95.1

Duplicates removed (%)

72.5

Number of peaks

286 (qval < 1E-05)