GSM1816442: NOMO1 115R2 Clock; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
anti-KAT13D/Clock (Abcam, ab3517, lot GR130170-5, 1mg/ml)
Sequenced DNA Library
Chromatin immunoprecipitation (ChIP) was performed as previously described (Kowalczyk, 2012) with minor modifications. Specifically, cells were first fixed in 2mM EGS (Thermo Scientific) for 30 minutes or 1 hour, followed by 1% of formaldehyde for 30 or 15 minutes at room temperature respectively. Chromatin was sonicated to a size less than 500bp and then incubated overnight with appropriate antibodies. ChIP sequencing (ChIP-Seq) libraries were prepared through the process of DNA end-repair (Epicentre), A-base addition and adaptor ligation using indexed Illumina adaptors followed by enrichment PCR. All enzymatic steps are carried out using enzymes from New England Biolabs. Final libraries were pooled, size selected and sequenced on HiSeq2500 with 25 paired-end reads.