Antigen

Antigen Class

TFs and others

Antigen

GATA2

Cell type

Cell type Class

Blood

Cell type

Erythroblasts

MeSH Description

Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Sample

source_name

Adult erythroid progenitor cells (ProEs)

tissue

Adult ProEs (A5)

developmental stage/tissue

adult bone marrow

cell type

primary adult ProEs (A5)

chip antibody

GATA2

chip antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq analysis using the Illumina HiSeq2500, 10-20 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer’s protocol. Raw ChIP-seq data were processed using the Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC, hg18) were retained for downstream data analysis.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31152236

Reads aligned (%)

40.0

Duplicates removed (%)

36.3

Number of peaks

1656 (qval < 1E-05)

hg19

Number of total reads

31152236

Reads aligned (%)

39.6

Duplicates removed (%)

37.6

Number of peaks

1825 (qval < 1E-05)