Antigen

Antigen Class

TFs and others

Antigen

NFE2

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

Adult hematopoietic stem/progenitor cells (HSPCs)

tissue

Adult HSPCs (A0)

developmental stage/tissue

adult bone marrow

cell type

primary adult CD34+ HSPCs (A0)

chip antibody

NFE2

chip antibody vendor

Santa Cruz Biotechnology

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq analysis using the Illumina HiSeq2500, 10-20 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer’s protocol. Raw ChIP-seq data were processed using the Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC, hg18) were retained for downstream data analysis.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

35142544

Reads aligned (%)

96.9

Duplicates removed (%)

47.6

Number of peaks

6268 (qval < 1E-05)

hg19

Number of total reads

35142544

Reads aligned (%)

96.1

Duplicates removed (%)

49.1

Number of peaks

6354 (qval < 1E-05)