Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
2-cell stage
NA
NA

Attributes by original data submitter

Sample

source_name
NT input ChIP-seq
strain background
B6D2F1 (C57BL/6 X DBA/2)
development stage
2-cell
genotype/variation
wild type
tissue
nuclear transfer embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
500-1000 cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to illumina TruSeq DNA LT Sample Prep Kit( FC-121-2001, FC-121-2002)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
14794367
Reads aligned (%)
95.0
Duplicates removed (%)
15.7
Number of peaks
166 (qval < 1E-05)

mm9

Number of total reads
14794367
Reads aligned (%)
94.9
Duplicates removed (%)
15.9
Number of peaks
132 (qval < 1E-05)

Base call quality data from DBCLS SRA