Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Adult female
NA
NA

Attributes by original data submitter

Sample

source_name
Female whole animal_wildtype
tissue
whole animal
gender
female
age
1-3 days
genotype
w1118
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
To identify KDM5 binding sites, ChIP-seq was carried out using HA-tagged KDM5 genomic rescue transgene flies with anti-HA (with or without paraquat treatment for 6 hours). Wildtype w1118 adults were used for KDM5 and anti-H3K4me3 ChIP-seq. Libraries were prepared using Tru-Seq adaptors and then size-selected (150 bp-350 bp) and purified by agarose gel

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
24891608
Reads aligned (%)
94.0
Duplicates removed (%)
16.4
Number of peaks
1933 (qval < 1E-05)

dm3

Number of total reads
24891608
Reads aligned (%)
94.4
Duplicates removed (%)
12.8
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA